FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487498
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC84990.1548793275186615No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG74160.13514355722772017No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG252.3919373E-455.9995254
CGGTTCG802.910383E-1143.7496269
GTCCTAC65900.032.8764841
TACGCGC802.4710134E-630.62473955
TATCGCG604.9869664E-429.16641827
TCCTACA76600.028.8770372
TCGTGCG1352.6011548E-1028.51879321
ACGATCG500.007213007727.999768
TCGACGG755.592185E-527.9997661
TCGCGTA500.007213007727.9997616
CTACAGT84400.027.5353074
CCTACAG79000.026.714963
GTAGGAC77200.026.295113
CGTACGG1606.548362E-1126.25121138
TACAGTG95650.026.1628725
TATGGCG68450.026.02607316
TCGCGGA2300.025.86934549
CGGACTA2850.025.78925152
GCGCGTA1101.0181539E-625.45432759
CCGTGAT24150.025.362127
Produced by FastQC (version 0.11.5)