FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5685682
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG305.8814604E-446.66652323
CGACGCG404.5971883E-543.7498722
TCGACCG400.002420269234.99989313
TCCAACG12000.032.66656528
CCAACGA10000.031.14990629
CAACGAA10450.030.1434530
TGTCGCG703.4933153E-529.9999123
GTCCTAC56500.029.7352071
AATCCCG11150.029.50663819
CGAATGT10450.029.47359733
AACGAAT10350.029.08203731
ATCCCGT11850.027.76362820
TCCCGTT12050.027.30282221
GATTTCG25800.026.99675641
ATCACGG26650.026.92299335
GTAGGAC65550.026.8572793
TATGGCG51400.026.69252616
CCCGTTT12650.026.56118622
AATCACG28100.026.40561734
CGTTTCC12350.026.35619524
Produced by FastQC (version 0.11.5)