FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241152
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC34180.10545633157593351No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG33410.10308063305886303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA350.001256179139.9995355
ACGCGTA753.545938E-837.33289716
TCGACGT400.00242021934.9995913
AACGTCG501.7112872E-434.99958830
GTCCTAC36200.031.4232541
CGTACGA450.004309202531.11170820
TTCGTCG604.9866113E-429.16632762
TCCAACG8050.028.69531628
GCGAGAA18500.028.37892220
TAGGACC22900.028.274784
TATGGCG32450.028.2585316
CGCTACG500.00721268827.99967245
GGCGAGA18400.027.3918319
CGAATGT6700.027.16386233
GTAGGAC42050.027.0508143
TCCTACA44500.026.505312
GCCGGTT3600.026.25050421
TCACGGA17700.026.10219636
TGGCGAG34050.025.90318118
CGAGAAA20550.025.88857321
Produced by FastQC (version 0.11.5)