FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8698143
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGA216730.2491681270358512No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC110710.12728004126857884No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG110090.1265672454453784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35950.041.9607164
TCTCGTA34700.039.74030763
ATCTCGT35550.038.6916662
AGCCGAG45000.036.24414452
CGTCTAG12250.034.8568531
CAGCCGA52100.031.17056551
TATGCCG50200.030.81647768
TCGTATG50100.030.59854965
GATTGAC55500.029.64058539
GCCGAGC55100.029.4734453
ATTGACA56450.029.20376840
ATGCCGT53250.028.45985869
CGTATGC56950.028.14726366
CGATTGA58900.027.87016138
GTATGCC58650.027.39107767
ACAGCCG60500.027.30555750
CATCTCG51000.027.03899261
TTGACAG61000.027.02545441
GCCGTCT22950.026.84073665
TATACTG28000.026.749785
Produced by FastQC (version 0.11.5)