FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15269868
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC83000.024.669171
TCGTCAA27750.024.08994560
GTAGGAC86150.022.5477453
CTATACT65450.021.9250014
TATACTG71400.021.7155555
GATTTCG30650.021.35470641
AAGTCGT30350.021.1036657
CGTCAAG30450.021.03435761
TATGGCG48000.020.92695416
TCACGTT35000.020.89987427
TTCGTCA31500.020.88896644
TACACTG120900.020.7566975
CGTCATT32150.020.46643846
CCTATAC54000.020.1572863
ATTTCGT33750.020.01547842
TTTCGTC33450.019.98571643
TAGGACC75200.019.9666334
CTACACT92200.019.8914184
AGTCGTC31550.019.6353858
TCGTCAT34250.019.4159445
Produced by FastQC (version 0.11.5)