FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19933849
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG36250.036.97887465
TCTCGTA36250.034.66174763
CTCGTAT38250.034.31332464
CGTATGC43800.031.56355566
CGATTGA49400.030.04231638
ATGCCGT46200.028.78753969
TATGCCG49800.027.47959768
ATCTCGT45750.026.393262
CCGATTG60600.024.20114537
CTATACT63000.023.4441684
TGCCGTC55250.022.55177170
CATCTCG57000.021.98225461
TATACTG70950.021.951825
GATTGAC68750.021.84133339
TCCGATT67450.021.74232136
CCTATAC62050.020.5315883
GTATGCC70200.020.24192867
TACACTG135100.020.1552055
CTACACT108500.020.1287964
TATACCG11500.019.7823775
Produced by FastQC (version 0.11.5)