FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5817306
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG9800.043.2152141
CTAGACG5750.036.521583
GCTCGAA8450.033.5501511
TTGACGG13600.031.1395731
GACGGCT14450.029.30783333
TAGACGC13000.027.730654
ATACTCG5350.027.4765176
TGACGGC15750.026.88877532
CGGCTAT16450.026.80862635
CTCGAAT8650.026.30046312
TAGACTG35200.024.7584175
CTACGGT9100.024.23066513
CGTCTAA5750.023.739641
CCTACGG9000.023.72211812
ACGGCTA18650.023.45854634
CCTATAC26400.022.5377833
CCCGCTA4100.022.19502465
GACGTAC2850.022.10535822
TCGTACG952.7833684E-422.1051715
ACTGCGA11150.021.9738
Produced by FastQC (version 0.11.5)