FastQCFastQC Report
Thu 9 Aug 2018
HGLH3BGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLH3BGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490240519
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG34094010.6954547549342815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA26943750.5496026737031093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23076510.47071812927809015No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG6643360.1355122586266681No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG5759070.11747437791856613No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC5725490.11678940801708804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT20417700.073.290081
AGCAGTG21006550.071.037082
CAGTGGT22944900.064.928344
AGTGGTA23512550.063.4535875
GCAGTGG23826550.062.633
GTGGTAT25093350.059.471536
GGTATCA25369900.058.845078
TGGTATC26166150.056.979357
GTATCAA32036350.046.59269
AGAGTAC24523150.034.15074518-19
CATGGGG17739300.034.0960424-25
GCAGAGT24928200.033.65970216-17
TACATGG25082000.033.17188322-23
CAACGCA25823050.032.54729512-13
ACGCAGA25994350.032.35001814-15
ATCAACG26712900.031.52266110-11
AGTACAT26688950.031.34940320-21
CCTCACT6611100.030.73441172-73
TGGGGGC7329250.030.19535426-27
CAGAGTA24804950.030.1590218-19
Produced by FastQC (version 0.11.2)