FastQCFastQC Report
Thu 9 Aug 2018
HGLH3BGX7_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLH3BGX7_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490240519
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCAT392599968.008313160259199No Hit
CCAGGGCA382025477.792613119357439No Hit
GCCACGCT369296847.532972606044422No Hit
GCTACCTG318210526.4909061504971195No Hit
GCCTTGGT317912366.4848242378757766No Hit
CTGCAAGC301853276.157248499486025No Hit
AATAATGG282870765.770040399292251No Hit
AAGGGTGA279517455.70163907647136No Hit
AAAGTGCT263976535.384633047844827No Hit
TGCTGTAA263051405.365762106660956No Hit
CGTTAATC246755055.033346703029253No Hit
CTGGACTC241036464.916698042252195No Hit
GTGTCATC236578774.825769409729268No Hit
AAACCTCA236075694.8155075080605485No Hit
TTACTCAG226132574.612686247584525No Hit
GGGGGGGG208624884.255561748048819No Hit
TGTAGAAG193596733.9490152791715696No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)