FastQCFastQC Report
Thu 9 Aug 2018
HGLH3BGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLH3BGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490240519
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG1192600.017.6053091
GACTAAC1199400.017.5939431
CAATGGA1259500.017.5030618
CTGCGGA1049350.017.4582841
GGGACCT1025150.017.3601781
GCGCGAT974300.017.3477421
CGGACTG1193450.017.3252261
ATGCGAT1259950.017.295281
ATAACGC1238150.017.2647171
TGGGCGT1117650.017.238131
ACTGATG1201500.017.211771
ACGAGGA1153700.017.1874621
GACTGCG1331100.017.1693521
GATGAAA1292100.017.123441
GACCTGG1278900.017.1014271
CTCGAAA1154700.017.0979881
TGACGGC1168850.017.0886961
TGGACGC1172800.017.0884571
ATGGGAG1165950.017.0663381
GGAAAGC1194950.016.9914421
Produced by FastQC (version 0.11.2)