FastQCFastQC Report
Thu 16 Aug 2018
HGLFVBGX7_n02_10xgenomics_antmb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLFVBGX7_n02_10xgenomics_antmb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78252124
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2784830.3558791579893729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5136750.085.405721
AGCAGTG5568700.078.658992
GCAGTGG6377100.068.6496353
CAGTGGT6490050.067.3810044
AGTGGTA6797350.064.436385
TGGTATC7047500.062.0103767
GTGGTAT7088750.061.792426
GGTATCA7225650.060.561398
GTATCAA7311050.059.8054589
CAACGCA7143150.033.8128212-13
GCAGAGT7140700.033.7408116-17
AGAGTAC7119150.033.71818-19
TACATGG7031450.033.66188422-23
ATCAACG7275800.033.2358310-11
ACGCAGA7308600.033.082314-15
CATGGGG3401550.032.96169724-25
CATGGGA2686650.032.6287324-25
AGTACAT7348000.032.50940720-21
CAGAGTA7120550.030.64723618-19
ACATGGG6868250.030.5833424-25
Produced by FastQC (version 0.11.2)