FastQCFastQC Report
Thu 16 Aug 2018
HGLFVBGX7_n01_10xgenomics_antmb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLFVBGX7_n01_10xgenomics_antmb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78252124
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTC1942050.248178567012443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG214400.018.2256621
GACTAAC208850.017.9756741
ACTGATG209900.017.9658241
CAATGGA189750.017.9608678
GATGAAA199200.017.7232881
GAATGAA194400.017.6421721
CGTGAGC181350.017.6142441
TGACTAG202050.017.5616741
CCGTGGA180200.017.4759981
GGGACCT187450.017.4389081
GACCTGG197850.017.4354131
CGGACTG151000.017.4050581
GGGAATG175650.017.3964541
ACTGAAC173350.017.3667091
CTGCGGA149100.017.3098241
GGAAAGC157600.017.2626971
TGAGCCG161150.017.1627031
GATGCTA174500.017.1439041
GACTGCG204250.017.1308021
TGACGGC129500.017.0582561
Produced by FastQC (version 0.11.2)