FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26403617
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3861141.4623526769078645No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1508340.5712626417812378No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA878850.33285212401013087No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA787980.29843638468168965No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA692120.2621307527676984No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG684960.2594190030858272No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA620430.23497916970996813No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA613150.23222197170940634No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC582050.2204432824487645No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA532220.2015708681125014No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA525630.19907499794441044No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA431530.16343594137121442No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT365250.13833332001445106No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA326720.12374062235488419No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT318690.12069937236250623No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA317060.12008203270029254No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT310660.1176581223701283No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG285690.1082010847225969No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG277150.10496667937578402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC158350.049.4664341
GCGTCCC20250.044.074131
GCGATCC40150.043.3250851
GCGGTCC14300.041.85321
GGCCCCA230050.039.6933673
TGCGACC109500.039.0274471
CGCGACC98150.039.0117651
GGCGACC130650.038.683551
GCCCCAG291900.037.949764
GCGGACC60950.037.7276951
GCGACCC872550.036.3177381
CGTACCC21450.032.4706572
GTCGACC25900.032.297341
CGGCCCC235900.032.2401542
GCGGCCT14550.031.7526191
GCGAGCC69300.031.0606421
GCGGGCC42400.030.9552291
CCCCCAG73800.030.9215074
GCGCGAC22350.030.6933162
GCCAGGT87150.030.4018756
Produced by FastQC (version 0.11.2)