FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24839898
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3262231.3133024942372953No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1261290.5077677855198922No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA785070.3160520224358409No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA558660.22490430516260573No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA534370.21512568207808258No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACC459580.18501686279066043No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA379720.1528669723200957No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA260660.10493601865836968No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA249250.10034260205094239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC153550.048.2776571
GGCCCCA213700.040.502413
TGCGACC106950.039.56541
GGCGACC118750.038.286491
GCGTCCC19800.037.8284571
CGCGACC86750.037.562131
GCCCCAG276100.037.345114
GCGGACC53500.036.897361
GCGACCC765800.035.2652441
GCGATCC49900.032.8959431
ACCCAGG115500.031.9093025
AATCGGC90750.030.50622770
ACAGGTC107000.029.864867
GCGGTCC18400.029.8642651
AGGTCAG2389400.029.8216139
CAGGTCA2334850.029.8070018
CCAGGTC2165650.029.7018477
GTCAGGC2452650.029.50595711
CCCAGGT1922300.029.4652166
GGTCAGG2464600.029.38886810