FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33994445
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA4223921.2425324196350316No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1545290.45457132775663783No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA1381580.4064134596108276No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA1244640.366130407482752No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG1199950.3529841419679009No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC920080.2706559851175685No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA880600.25904232294423396No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA663770.19525837236054303No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA600660.1766935744943034No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA598080.17593462696625875No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA550010.1617940813565275No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT520710.15317502609617542No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG510910.1502922021524399No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA469260.13804020039156398No Hit
CTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTT399770.11759862530481083No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA370800.1090766447282784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC168750.046.9165761
GCGATCC50950.042.7977871
GCGTCCC24600.038.273191
GCGGTCC17550.037.8925551
TGCGACC133400.037.7294581
GGCCCCA252400.037.2321133
CGCGACC113000.036.14681
GCGACCC967000.035.7174221
GCGGACC74350.035.5892031
GGCGACC162500.035.3884621
GCCCCAG319150.035.3780754
ACCCAGG142250.031.5429085
CGTACCC24800.030.2012022
AGGTCAG2971800.029.9256469
CAGGTCA2914300.029.8572838
GTCAGGC3033500.029.80605311
GGTCAGG3039550.029.74455510
CCAGGTC2695950.029.6700527
TCAGGCG3055050.029.61748712
CCCAGGT2401700.029.411156
Produced by FastQC (version 0.11.2)