FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33550065
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA5155561.5366766055445795No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1861190.554750042958188No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1144340.34108428702001026No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA965310.2877222443533269No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA935850.2789413373714775No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG872250.25998459317440964No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA868790.25895329860016664No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA810580.2416031086675987No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC722760.2154273024508298No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA641890.19132302724301728No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA574930.1713647946732741No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA447320.1333291008527107No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA440930.1314244845725336No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT384240.11452734890379497No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT381610.11374344580256401No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG356870.10636939153471088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC195800.052.661671
GGCCCCA282250.042.4461023
GCGGACC70200.042.229981
GCGATCC46150.042.01581
TGCGACC163450.039.1012341
GCCCCAG367300.038.964014
GCGGTCC18900.038.704291
GCGTCCC27200.037.188061
GGCGACC189450.036.0443531
CGCGACC146100.035.9108771
GCGACCC1208000.034.4384351
GCGAGCC73700.034.0507161
CAAGGAT1957700.033.9516470
ACCCAGG135600.032.780245
GCGGGCC51250.032.2346341
CGGCCCC315800.032.1070182
CAGGTCA3315850.029.904678
CCCCCAG113350.029.8896834
AGGTCAG3373650.029.8521399
CCAGGTC3117450.029.7397147
Produced by FastQC (version 0.11.2)