FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18145356
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA2127421.1724322190206684No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG794070.4376161040874591No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA501090.27615330335761945No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA457770.25227942620690386No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA423970.23365207053529288No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG423210.23323323058528034No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA402710.22193557403888906No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA371900.20495602290745907No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC351840.19390085264791718No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA297160.1637664204549087No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA288220.15883953999028733No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACC211540.11658079345481015No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA190440.10495247378998793No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT181530.1000421264812881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC101400.045.2513121
TGCGACC79450.041.1011731
GCGGACC34200.040.219151
GGCGACC81100.039.8765531
GGCCCCA134750.039.0642553
GCGTCCC14100.036.7374571
CGCGACC62150.036.548541
GCCCCAG176500.035.7332884
GCGATCC28450.035.3074261
GCGACCC510850.033.7563631
GCGAGCC43800.030.9245451
GCGGGCC26650.030.4689331
CGAACCC37450.030.3733022
GCGGTCC10500.029.6665571
CAGGTCA1533400.029.1521078
CCAGGTC1422500.029.052737
AGGTCAG1570150.029.0459339
GTCAGGC1603900.028.83400711
CCCAGGT1270450.028.7804056
ACCCAGG81000.028.7343865
Produced by FastQC (version 0.11.2)