FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14971453
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA415580.27758160814451344No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA226740.15144822616749357No Hit
GAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCC215660.14404747488436828No Hit
GGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACC207910.1388709566132292No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA190260.12708185371186084No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG189170.12635380146469416No Hit
GTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACG181580.12128415324818506No Hit
GTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAA180800.12076316173186397No Hit
GGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGCCGCACC172940.1155131702981668No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG165700.11067730032616073No Hit
CGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTC162200.10833951788113017No Hit
CGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGG149920.10013724118828013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC27900.035.12671
GCCCCAG43400.032.4999664
GCGACCC105500.031.7831251
GGCGACC21900.030.8458481
TGCGACC16700.030.5998941
GGCCCCA38850.030.4501153
GTCAGGC352300.029.11900911
CCAGGTC306300.029.035527
CAGGTCA341450.028.8551438
TCAGGCG358400.028.65260112
CCCAGGT268150.028.6240656
AGGTCAG351950.028.5118839
GGTCAGG364650.028.20027210
CCCCAGG219800.028.0096195
TACCCGC374750.027.2064723
CCCGCTG376300.027.04708725
ACCCAGG19800.027.0455175
TTACCCG378300.026.94191422
CAGGCGG373600.026.9247613
CGACCCC131850.026.8369262
Produced by FastQC (version 0.11.2)