FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27682512
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA5175061.869432947414599No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1988160.7182007182007182No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1265640.4571983929782095No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA888190.32084877268363504No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA887420.3205706187357564No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA664600.2400793685197355No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACC512910.18528304078762795No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA502520.181529768685732No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA486440.1757210472806803No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG412330.14894963289458701No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA402690.1454672899627028No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA395470.14285914515272313No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG375840.13576802567628257No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC371290.1341243887115447No Hit
GCGCCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA319340.1153580282020649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC220050.051.295591
TGCGACC168250.044.101411
GCGGACC68100.042.0928651
GGCCCCA311750.041.146133
GCGATCC50200.040.7174721
GGCGACC189550.039.6442261
CGCGACC136750.039.3129431
GCGTCCC28650.038.3598331
GCCCCAG402750.038.097644
GCGGTCC22150.036.0273861
GCGACCC1195850.035.3998031
GCGAGCC78500.035.178641
GTCGACC31100.033.9874231
GCGGGCC52550.032.6358571
ACCCAGG142550.032.3847545
CGAACCC69700.032.0869752
GTGACCC60000.031.9669341
CGTACCC27650.031.8981952
GCGGCCT16850.031.157531
GTACCCC27400.031.0396773
Produced by FastQC (version 0.11.2)