FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26009764
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3347311.2869436262474354No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1244290.47839342179344646No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA931300.3580578432007303No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA927340.3565353380369003No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA873900.33598920774521446No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG810030.311433044913441No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC691770.26596550433906285No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA670580.2578185638285684No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA542440.20855244976463455No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT534930.20566507254737107No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA527980.20299299909064922No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA481760.18522274942594635No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT413280.1588941752797142No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT388320.14929777909557349No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA378280.14543769024586303No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA371570.1428578898293733No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA331240.12735217436036714No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG327590.12594885520683693No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA297720.11446470640794741No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA277600.10672914986848785No Hit
GAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCC263740.1014003817950828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC133550.046.8856961
TGCGACC91700.043.168521
GCGGTCC12500.042.840581
GCGATCC31350.041.6433031
GCGTCCC18150.039.5322151
GGCCCCA190750.038.7155233
GGCGACC113150.038.696941
CGCGACC79350.038.5071371
GCCCCAG239450.037.199914
GCGACCC726500.037.192521
GCGGACC51150.035.171541
ACCCAGG97850.032.013415
CGTACCC17900.031.6758562
GTGACCC41100.031.4237841
CGTCCCC22150.030.8125252
CGGCCCC199650.030.7311652
CCCCCAG63700.030.65944
CGACCCC938250.030.6484322
CAGGTCA2194750.030.610618
AGGTCAG2242000.030.5622489
Produced by FastQC (version 0.11.2)