FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26374872
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA4039981.5317534052866684No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1466050.5558510388221031No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA972740.36881316428758404No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA926100.35112966614586794No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA855150.32422906166141774No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG833640.31607357184520174No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC731580.2773776494536163No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA696600.2641150258473292No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA687210.26055481899589883No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA659330.2499841515818541No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA478680.1814909281834619No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT452900.17171647316430577No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT399270.1513827251938891No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA389900.1478301013176481No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT376510.14275329942833467No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA351450.13325183151599748No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA335470.12719303433965481No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA325420.12338258930697368No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG271480.10293130522112107No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA265110.10051612762329236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC151550.051.109641
GCGATCC37350.042.3571
GCGGTCC15500.042.2267071
GCGGACC54400.041.563391
TGCGACC124900.040.7454721
GGCCCCA228950.039.4099243
GGCGACC143950.039.3165741
GCGTCCC22050.039.207191
CGCGACC102450.038.8783341
GCCCCAG291950.037.283664
GCGACCC917300.035.0846941
GCGAGCC58600.034.0450971
ACCCAGG114400.032.705625
CGTACCC22300.032.3313642
GTCGACC29450.032.0889741
GCGGGCC39150.031.8269921
CAAGGAT1026000.031.66992470
CGAACCC58100.030.9031622
GTGGCCC15750.030.889551
ACAGGTC114450.030.8869827
Produced by FastQC (version 0.11.2)