FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21612134
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGC311550.14415513063170904No Hit
TTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAA260270.12042771898415955No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCG251570.1164022025774965No Hit
TGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAG240180.11113201500601466No Hit
TGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCA230400.10660677932128314No Hit
GGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCAT217970.10085538059314272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC27300.036.40960770
GCGGCCC10700.034.0192641
GCGGGCG46350.033.45214
AGCGGGC23050.032.3425333
CTGCGCG12750.028.5485170
TACCGCG238550.027.89099361
CGAGGTA247200.027.18404466
GGCGGGC35250.026.8083423
ACCGCGA252100.026.50295862
CCGCGAG254000.026.40116563
AGTACCG256050.026.35381959
CGCGAGG257800.026.05273864
GCGAACA262500.025.86632552
CGATAGC263150.025.76265147
CAAGCGG16700.025.3597091
CCGATAG269650.025.18057446
GGCCCCA16150.025.1391583
GATAGCG271100.025.04577648
GAGGTAA271150.024.93778267
GCGAGGT276700.024.27320565
Produced by FastQC (version 0.11.2)