FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183227
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA12750.6958581431775884No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG4750.2592412690269447No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3050.16646018326993292No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA2260.12334426694755685No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA2200.12006964039142703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGG150.002221912170.04
CTCAGGT150.002221912170.06
GCGAGCA150.002221912170.01
GCGGCCC453.8198777E-1062.2222181
CGACACC252.3857168E-456.0000042
GCGGACC252.3857168E-456.0000041
ACCGGTC200.006942760752.4999965
GACGGGC200.006942760752.4999963
GGCGCGC200.006942760752.4999967
GGCCTGG200.006942760752.4999961
TAGTCGG200.006942760752.4999965
GGTAGGT200.006942760752.4999961
TGCGACC651.8371793E-1048.4615361
ATAGAAG305.8664003E-446.66666414
ACAGGTC350.001253008340.08
GGACCCC350.001253008340.03
GGCCCCA801.4115358E-939.3749963
GCGACCC4000.035.8750041
GGCGACC501.7048874E-435.0000041
GTGCGAC400.002414147134.9999961
Produced by FastQC (version 0.11.2)