FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29791162
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA991850.33293431118933864No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG320830.10769301311576904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC37900.048.206231
GGCCCCA58500.037.7517663
GCGGACC19900.035.1761861
CGGCCCC51900.034.9320452
GCCCCAG73100.034.66484
GCGGGCG73450.034.5949174
GCGACCC280050.033.281851
AGCGGGC37000.031.8780693
ACCGAGC27000.031.49943270
CCAGGTC660250.029.457947
GTCAGGC729000.029.4168811
CCCAGGT593300.029.2424856
TCAGGCG732400.029.23720712
CCCGCTG730150.029.04825225
GGCGGGC53100.029.0016023
CCCCAGG518600.028.9935515
AGGTCAG728350.028.9868559
CAGGTCA716800.028.9747668
CCGCTGA733050.028.92383426
TACCCGC736350.028.89009723
Produced by FastQC (version 0.11.2)