FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31589718
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCG61950.033.389594
AGCGGGC30050.032.8447653
GGCGGGC40300.030.1360243
CAAGCGG24650.027.6876471
TACCGCG330500.026.99337261
CGAGGTA340950.026.02219866
AGTACCG349800.025.71414659
GTCGGGC41900.025.5604743
ACCGCGA355150.025.22823762
CCGCGAG355000.025.13044763
CGCGAGG360800.024.69736564
CGATAGC367350.024.51459347
GATAGCG369700.024.46282648
AAGCGGG31700.024.4001852
CCGATAG375450.024.06972546
GCGAACA374950.023.998852
GAGGTAA380950.023.43684667
GAGGGCG96950.023.4294364
CTGCGCG5700.023.3327670
ACCGATA387100.023.06515345
Produced by FastQC (version 0.11.2)