FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31358325
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCG51550.032.725654
AGCGGGC26000.030.6920993
GGCGGGC36750.027.999813
TACCGCG295450.026.25100961
AGTACCG312800.025.11943259
CGAGGTA307900.024.91661366
GCGGCCC12250.024.8574491
ACCGCGA317350.024.54970262
CCGCGAG315600.024.49730363
ACCGAGC25650.024.42439570
CGATAGC326950.024.09675247
CGCGAGG319600.024.08119264
GATAGCG329150.023.97815148
CCGATAG331850.023.76204146
GCGAACA335450.023.60074652
GTCGGGC39200.023.2141283
GAGGGCG94650.022.6678224
ACCGATA350050.022.27666545
GCGAGGT343400.022.22870865
CCCGCTG174900.022.13210925
Produced by FastQC (version 0.11.2)