FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_lex01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_lex01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30053947
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA619000.20596296386627688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC27250.037.3760641
AGCGGGC36850.036.471623
GCGGGCG79100.036.017564
GCGACCC173100.032.4320451
CAAGCGG27300.032.307621
GTCAGGC433150.028.33816711
CCAGGTC392600.028.2960537
GTCGGGC55200.028.2151413
GGCGGGC54900.028.1143173
TCAGGCG433850.028.04230712
CCCAGGT358600.027.9531356
CAGGTCA431000.027.6183598
CCCCAGG318600.027.5627125
TACCGCG517650.027.49774661
AGGTCAG440050.027.4087359
TACCCGC440150.027.4021923
CCCGCTG440100.027.38080625
CGACCCC214050.027.3062782
CCGCTGA443800.027.19196126
GAGGGCG132550.027.038754
Produced by FastQC (version 0.11.2)