FastQCFastQC Report
Tue 21 Dec 2021
HGL5JAFX3_n02_as1-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGL5JAFX3_n02_as1-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988354
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG347060.6957405188164272Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT229660.4603923458519584Illumina Single End PCR Primer 1 (100% over 31bp)
CGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT141670.28400149628514737Illumina Single End PCR Primer 1 (100% over 33bp)
TCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA137510.27566207209833143Illumina Single End PCR Primer 1 (100% over 34bp)
CGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG104530.20954807938650707Illumina Single End PCR Primer 1 (100% over 32bp)
CGTCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG67180.1346736819399746Illumina Single End PCR Primer 1 (100% over 30bp)
TCGTGTAGGGAAAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT62060.12440977524850884Illumina Single End PCR Primer 1 (100% over 33bp)
CGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG53600.1074502731762822Illumina Single End PCR Primer 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)