FastQCFastQC Report
Tue 21 Dec 2021
HGL5JAFX3_n02_as1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGL5JAFX3_n02_as1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4816333
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG104600.2171776743842255Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT67280.13969133778748272Illumina Single End PCR Primer 1 (100% over 31bp)
TCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA53380.11083120706147187Illumina Single End PCR Primer 1 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)