FastQCFastQC Report
Tue 21 Dec 2021
HGL5JAFX3_n01_as1-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGL5JAFX3_n01_as1-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988354
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC347510.6966426199904818Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT230400.4618758011159593Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA142070.28480336399541817Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC137910.2764639398086022Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC104880.21024971363299397Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG69030.13838232009997686Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA62120.12453005540504945Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG53750.10775097356763373Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA50590.10141621865649472Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)