FastQCFastQC Report
Tue 21 Dec 2021
HGL5JAFX3_n01_as1-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGL5JAFX3_n01_as1-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7537977
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC119420.15842446852782915Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT88610.11755143322936645Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)