FastQCFastQC Report
Mon 6 Aug 2018
HGKVLBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKVLBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511966226
Sequences flagged as poor quality0
Sequence length99
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28255920.5519098441466332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG25244720.4930934643333289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA23624290.4614423530352176No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG9089960.17754999330756635No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG6436200.12571532404170738No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT5481860.10707464128698208No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC5136750.10033376693875896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG17005550.070.547152
AAGCAGT17416750.069.124321
CAGTGGT18613350.064.336474
AGTGGTA18981800.063.166715
GCAGTGG19411400.061.8187983
GTGGTAT20021250.059.8997466
GGTATCA20372750.058.91018
TGGTATC20812850.057.5790757
GTATCAA26180700.045.8340159
CATGGGG14187050.033.79583724-25
AGAGTAC19962450.033.40625818-19
GCAGAGT20181450.033.09549716-17
TACATGG20337350.032.55387522-23
CAACGCA21045050.031.7772412-13
ACGCAGA21116400.031.68013414-15
ATCAACG21486700.031.16425310-11
AGTACAT22053650.030.20999720-21
CGCAGAG20207500.029.86901516-17
CCTCACT5077000.029.56289572-73
CAGAGTA20392350.029.52140418-19
Produced by FastQC (version 0.11.2)