FastQCFastQC Report
Mon 6 Aug 2018
HGKVLBGX7_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKVLBGX7_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511966226
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA400629427.825309554697071No Hit
CCTGTGCG365103407.13139620268623No Hit
CAACGCCT359175357.015606338063401No Hit
GGACACGT344154576.7222123750014715No Hit
CTAGACCT337752216.597158032061278No Hit
TACTCTTC328708836.420517864395219No Hit
TGTGCGGG325707526.361894661387292No Hit
GAGATGAC318383496.21883776372389No Hit
AGTTCGGC308720976.030104220195182No Hit
TCCGGAAG292197245.70735382845352No Hit
ACTTCATA292120355.705851971571265No Hit
ATGAGAAA261158205.101082585865732No Hit
ACCATAAC253110384.943888232189754No Hit
TGCCGTGG235461224.599155335688101No Hit
GTAATCTT233784724.56640903495849No Hit
GGGGGGGG186217203.637294621071352No Hit
GTGTATTA128645312.5127694653826635No Hit
CTAGCCCT5124070.10008609435107542No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)