FastQCFastQC Report
Mon 6 Aug 2018
HGKVLBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKVLBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511966226
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1460100.017.8763898
GACTAAC1367500.017.8155781
CTGCGGA1131850.017.7710381
GGGAATG1204950.017.5916481
GGGACCT1178400.017.4852851
GCGCGAT996500.017.4042471
TGACGGC1272200.017.370111
CTCGAAA1266800.017.3413411
GAATGAA1341800.017.3160921
GACCTGG1445850.017.30711
GATGAAA1374050.017.2811761
ACTGATG1268450.017.2674451
ACGAGGA1233550.017.2578641
TGACTAG1369700.017.1382141
ATAACGC1234900.017.1173671
CGGACTG1148850.017.0774571
ACTGAAC1299600.017.0588471
GGCTCGA1287600.017.0579451
CGTGAGC1124000.017.0475851
TGGGCGT1109350.017.0379031
Produced by FastQC (version 0.11.2)