FastQCFastQC Report
Wed 8 Aug 2018
HGKTKBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKTKBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514832185
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG35992300.6991074188572729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA27600070.536098379319467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24175380.4695778683688938No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG7008820.136137953379896No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC5862860.1138790497334583No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG5747820.11164453519936791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT21369700.073.31081
AGCAGTG21992000.071.037112
CAGTGGT23963950.065.071364
AGTGGTA24555500.063.613595
GCAGTGG24952400.062.6190343
GTGGTAT26213000.059.6120646
GGTATCA26479500.059.0324448
TGGTATC27308000.057.1750957
GTATCAA33521750.046.5946589
AGAGTAC25668300.034.18523418-19
CATGGGG18547800.034.17373724-25
GCAGAGT26057500.033.7266916-17
TACATGG26246300.033.1920122-23
CAACGCA26967350.032.63130612-13
ACGCAGA27178400.032.4130414-15
ATCAACG27938700.031.54831910-11
AGTACAT27928250.031.37576920-21
CCTCACT6968200.030.84442772-73
CAGAGTA25934750.030.22689618-19
TGGGGGC7776950.030.12303726-27
Produced by FastQC (version 0.11.2)