FastQCFastQC Report
Wed 8 Aug 2018
HGKTKBGX7_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKTKBGX7_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514832185
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCAT414101498.04342661677222No Hit
CCAGGGCA402687867.821730492626447No Hit
GCCACGCT388555037.547217157761805No Hit
GCTACCTG334980506.506595930866288No Hit
GCCTTGGT334948176.505967959248701No Hit
CTGCAAGC317339856.163947384136445No Hit
AATAATGG298117135.790569018135492No Hit
AAGGGTGA294064715.71185560203467No Hit
AAAGTGCT277744735.394859491933279No Hit
TGCTGTAA276751695.3755708765566No Hit
CGTTAATC260140935.052926712419892No Hit
CTGGACTC254264444.9387829162234675No Hit
GTGTCATC249577934.847753059572217No Hit
AAACCTCA248296114.822855237770343No Hit
TTACTCAG238111044.625022423568954No Hit
GGGGGGGG210016174.079313145505851No Hit
TGTAGAAG203769913.9579870089124287No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)