FastQCFastQC Report
Wed 8 Aug 2018
HGKTKBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKTKBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514832185
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG1253950.017.7342071
GACTAAC1266900.017.6292021
CTGCGGA1087850.017.5108031
CAATGGA1327050.017.4828138
GCGCGAT1025100.017.4003411
CGGACTG1253000.017.3553641
ATGCGAT1324600.017.270411
ATAACGC1302150.017.2479521
TGACGGC1245700.017.2403851
ACTGATG1259350.017.2319931
GGGACCT1080500.017.2061061
GACTGCG1397000.017.1715411
GATGAAA1370300.017.1481861
TGGGCGT1166900.017.1193181
ACGAGGA1204800.017.094251
GACCTGG1343600.017.0660531
ATGGGAG1224450.017.0017681
CTCGAAA1206600.016.9860571
GGAAAGC1255150.016.9532661
TGGACGC1219900.016.9239831
Produced by FastQC (version 0.11.2)