FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25149694
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2354430.9361664599179617No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG642470.25545837655122167No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC510730.2030760294737582No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA476030.18927864490120636No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG267560.1063869802948696No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA259300.10310264609978953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA589450.069.509821
AGTAGGT423700.069.447667
GGTAGTA587500.069.282154
TAGGTTG424150.069.274979
TAGTAGG424950.069.2517556
GTTGTAT328750.069.2058812
TGTATAG430850.069.0507214
TATAGTT394750.068.96866616
GTAGTAG586100.068.952065
GGCATAG154650.068.9513113
TTGTATA434700.068.9134713
CGGAGTC417000.068.8767514
AAGATGC156300.068.781945
TCCACCG419700.068.742049
AGGTAGT591950.068.68073
TAGTTAA392450.068.56132518
GTATAGT432650.068.540515
GCATAGC154350.068.5191714
GAGGTAG617300.068.518922
TCATTCC420700.068.518645
Produced by FastQC (version 0.11.5)