FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535937
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG572421.2619663809263664No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA339650.7487978779246713No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC302610.667138895447622No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG249880.5508894854580212No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA171120.37725391688641174No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT151090.3330954552499296No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC146380.3227117131476914No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG137890.3039945219697716No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA135350.2983947969294988No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAACTGTAGGCACCATCAAT130300.2872614853336808No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA79170.1745394611962203No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC76040.16763901262297073No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA74910.1651477963648966No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC68600.15123666841051805No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA59660.13152739996168378No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA59080.1302487225902829No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA51390.11329522433843327No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC49940.11009853090993106No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT48640.10723252990506701No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA48150.10615226798784903No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT46580.10269102062043631No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG46200.10185326648055298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGGC114700.069.507529
GCTGGCA114700.069.38547510
CGGAGTC247900.069.3619514
CAAGATG114900.069.356074
AGTAGGT264900.069.321927
TGAGGTA374200.069.313551
GGTAGTA367450.069.290914
GTAGGTT264600.069.268258
AGATGCT115300.069.2672356
GTTGACA156800.069.2421918
GGCATAG115100.069.2370813
AGGTTGT275500.069.20811510
GGAGTCT248250.069.19672415
CCTTCAT248600.069.1860662
TATAGTT239800.069.1699316
GCTTATC253550.069.157543
AAGATGC115400.069.146565
TAGGTTG264850.069.110379
TAGTAGG266000.069.02216
GTTGTAT201800.069.0079412
Produced by FastQC (version 0.11.5)