FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7560773
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG990771.310408340522854No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA836641.1065535230326318No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC694150.9180939567951584No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA462720.6120009157793787No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG429190.5676535983820702No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC343920.45487412464307553No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA315970.41790700501126005No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT292510.3868784316101012No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA217670.2878938436585783No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC174500.23079650718253278No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG172220.2277809425041593No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC170580.2256118521214696No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA164180.21714710916463173No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA141550.18721630711568776No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA126730.16761513670626008No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAACTGTAGGCACCATCAAT122390.16187498288865437No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA121040.16008945117119638No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT116220.15371444163182785No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT112740.14911173764904725No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC112630.1489662498794766No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA106180.14043537611828844No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG92860.12281812983936959No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG89480.1183476874652896No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC84820.11218429649984202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT528700.069.668732
AAGATGC197400.069.587785
AGATGCT198000.069.483436
TGCTGGC198150.069.46579
GTAGGTT507400.069.443768
CAAGATG198050.069.3594064
GGAGTCT529850.069.3396215
AGGTTGT529750.069.3349210
AGTAGGT509750.069.32967
GGTAGTA703850.069.279554
TAGGTTG508150.069.27249
GCTGGCA197900.069.2704910
AGCTTAT471850.069.265392
CGGAGTC532350.069.2347914
TAGTAGG510000.069.23436
TGAGGTA718700.069.209761
GTTGACA302400.069.1913718
GCTTATC472500.069.161323
GTTGTAT395000.069.1454412
CTTCATT532350.069.117363
Produced by FastQC (version 0.11.5)