FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842518
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1268971.435077655482296No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC729440.8249233985161241No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG547270.6189074198096062No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA398150.45026767262447187No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT366140.4140675766789505No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC363810.4114325806291828No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA324950.3674858224772627No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG227930.25776594404444525No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA222390.2515007603037958No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC212780.24063281522299418No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC187680.21224723545940194No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA144850.16381080592654718No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC141870.1604407251418657No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA141760.16031632618672645No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA136800.1547070642095385No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT135760.1535309286336765No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT134420.15201552318016204No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA124580.14088747119316014No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT116830.13212299935380398No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAACTGTAGGCACCATCAAT115020.13007607109196725No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC114680.12969156523062775No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG108840.1230871116123258No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA107770.12187704904869857No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT101040.1142660947933609No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG93780.10605576375417047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT644550.069.600662
CGGAGTC645100.069.52425414
GTAGGTT577100.069.467468
AGTAGGT578250.069.426157
GGAGTCT644900.069.4187115
TGAGGTA818750.069.3862461
TAGGTTG577950.069.371359
AAGATGC234650.069.367075
AGATGCT234550.069.3534556
CCACCGG647800.069.3467610
GGTAGTA803300.069.331274
AGCTTAT590950.069.327812
AGGTTGT603400.069.30485510
TGCTGGC235300.069.296019
TAGTAGG578200.069.286886
GCTGGCA235250.069.2809810
CTTCATT647500.069.233353
TATAGTT532600.069.22632616
CCGGAGT649450.069.2198613
GCTTATC591850.069.2093
Produced by FastQC (version 0.11.5)