FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10651685
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA1966401.8460928951616575No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1195951.1227801047439911No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1022970.9603832633052892No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC694400.6519156358829613No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG470140.4413761766330867No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA441060.4140753317432876No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA358400.3364725862621736No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA332280.3119506444285576No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT331010.31075834480647896No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA303020.2844808121907473No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC277550.26056910244717146No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG223720.21003249720584113No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC219220.20580781350556274No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC188590.1770517997856677No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT154030.14460622896752956No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA151820.1425314398613928No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC148590.139499055783193No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA123410.11585960343363515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT561800.069.5615842
GTAGGTT633150.069.481638
AGTAGGT635050.069.433577
AGGTTGT663950.069.42641410
TGCTGGC220800.069.361689
TGAGGTA906600.069.3537141
AAGATGC220700.069.3432545
TAGGTTG634250.069.3169869
CTTCATT563750.069.301373
GTTGTAT488800.069.27286512
GGTAGTA887150.069.251764
TAGTAGG636450.069.214846
GGAGTCT562700.069.1779515
GGTTATC355800.069.1733317
AGCTTAT557400.069.162572
TATAGTT585400.069.1503316
AGATGCT221350.069.126086
CAAGATG221850.069.04694
TGTATAG648900.069.0248814
GCTTATC558800.068.994583
Produced by FastQC (version 0.11.5)