FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10745492
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1201781.118403885089673No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC806990.751003304455487No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA694390.6462151756289987No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA472360.4395889922955598No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG458320.42652304799072954No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC406280.3780934367639937No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA346200.32218161811483365No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT318720.2966081031934136No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA239870.2232284943304597No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC212070.19735718010864461No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG182120.1694850268373007No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA171410.15951805650220577No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC169760.15798252885954406No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA163030.15171943732311186No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT155410.1446280914824561No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA138160.12857484794553847No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA122910.11438285003608954No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC122270.11378725143529955No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA118150.10995308544271402No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG111010.10330843855265073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT649550.069.472987
CCTTCAT576200.069.442892
GTAGGTT648950.069.4077768
TAGGTTG650000.069.27959
GGTAGTA911700.069.275514
TGCTGGC237350.069.243619
TGAGGTA933400.069.242841
AGGTTGT682650.069.2317310
CGGAGTC576350.069.2295314
AAGATGC237650.069.1833955
TAGTAGG651550.069.1791466
AGATGCT237600.069.1707466
AGCTTAT541800.069.168792
CTTCATT578500.069.159783
TATAGTT602300.069.09849516
GGAGTCT576500.069.0693615
GCTTATC542100.069.052083
GTTGTAT504150.069.0445712
GCTGGCA238300.069.02630610
CCGGAGT579950.068.9989313
Produced by FastQC (version 0.11.5)