FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8007944
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG978161.2214870633460975No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA750290.9369321263984863No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC660320.8245811908774587No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA514350.6422996964014733No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG396110.4946463162080054No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC344830.4306099043649656No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA294890.36824683089691934No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT275800.3444080028531668No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA225350.28140806179463795No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC169740.21196451923240225No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA165130.2062077357184316No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC154060.19238396272501407No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA134430.1678708042913387No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG132270.16517348273164748No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA123570.15430927089400226No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA116070.14494357103396327No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT113920.14225873707408543No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC106810.1333800536067685No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA106730.1332801528082614No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT97540.1218040485797603No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG94130.1175457770433959No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG93550.11682149625421956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT523900.069.5833052
GTAGGTT555800.069.4865658
AGTAGGT557250.069.481627
TAGGTTG556400.069.405349
TGAGGTA784650.069.3383941
TGCTGGC203200.069.307689
GGTAGTA768850.069.297574
TAGTAGG557950.069.294086
AGGTTGT582650.069.2938310
TATAGTT510050.069.2607416
GGAGTCT524400.069.24721515
CTTCATT526100.069.2191543
AGCTTAT437400.069.2053452
AAGATGC203250.069.2019355
AGATGCT203400.069.1879046
GTTGTAT437450.069.18112
CGGAGTC527250.069.12662514
GCTGGCA203650.069.0857910
TTCATTC527550.069.025454
GTTGTGT152800.069.0121512
Produced by FastQC (version 0.11.5)