FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4853571
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG615201.2675203473895817No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC420900.8671965445648163No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA305630.6297013065225583No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG211070.4348756822553951No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC197070.4060309409298844No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA189010.3894246112810547No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA164860.33966743249454884No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT149930.30890657620955786No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC105930.21825167490081016No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG97420.20071819285223189No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA85620.17640619659215864No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA80990.16686682856807905No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA79720.164250198462122No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC74900.15431936609148192No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT68380.14088595798845838No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA65710.1353848537499503No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC58390.12030317471404045No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA58390.12030317471404045No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG54700.11270052503610228No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG50060.10314055362536162No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT50030.10307874346537838No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA49010.10097719802594832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT302650.069.4991152
GTAGGTT319050.069.490338
AGTAGGT320000.069.459027
AGGTTGT335200.069.4415610
TGAGGTA454800.069.406851
TGCTGGC123300.069.398869
TAGGTTG319300.069.370169
TAGTAGG319800.069.327366
CAAGATG122900.069.311494
AGATGCT123450.069.286196
GGTAGTA444350.069.246694
AAGATGC123300.069.171785
GTTGTAT252600.069.1657712
GGATAGG57650.069.1478714
TATAGTT299800.069.14773616
GCTGGCA123900.069.1475210
AGCTTAT277500.069.1009142
CGGAGTC303700.069.0989214
GGAGTCT302550.069.0976415
TGTATAG332900.069.0936614
Produced by FastQC (version 0.11.5)