FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9051299
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1305391.4422128801622838No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1143781.2636639227142976No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC497930.5501199330615418No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG458860.5069548580816963No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG359390.39705902986963526No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA353640.39070635054703196No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT317240.350491128400465No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC282480.3120878008780839No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA224150.2476440122020055No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA195230.21569279724379894No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA192620.21280923323823464No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT186120.20562794356920483No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC158560.17517927537251834No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG143280.15829772058132208No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA129490.1430623383450265No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA126680.13995781158041515No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG126440.13969265626955865No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC118380.13078785707996168No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA113190.12505387348269018No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT112470.1242584075501207No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG99100.10948704710782398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT660450.069.600172
CGGAGTC662100.069.4991814
GTAGGTT645300.069.431258
TGCTGGC255950.069.4073269
AGTAGGT646950.069.373187
CCACCGG665200.069.34289610
GGTAGTA903850.069.3358844
AGATGCT256450.069.326596
TAGGTTG646650.069.3025369
AAGATGC256650.069.298685
GCTGGCA256200.069.29861510
AGCTTAT611700.069.293852
GGAGTCT662000.069.2744615
TGAGGTA928550.069.262731
AGGTAGT903850.069.259983
CTTCATT663400.069.2465443
AGGTTGT682050.069.2461310
TATAGTT603000.069.24071516
CCGGAGT665600.069.2276113
CAAGATG257400.069.219134
Produced by FastQC (version 0.11.5)