FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7851234
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC800051.0190117884653547No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG761780.9702678585302642No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC398500.5075635244090293No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG350040.4458407429965786No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA346850.4417776874310459No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA256870.32717149941015644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG245340.3124859098582465No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT240630.3064868528947169No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA215400.2743517770582306No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC196580.2503810229067176No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA141740.1805321303632015No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC141290.17995897205458403No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT127340.16219106448744233No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA126110.16062443177722124No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT106240.13531630823893415No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG102830.13097304194474396No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA100470.1279671450373279No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA97630.12434987926738651No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC95840.12206998288421922No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG86410.11005913210585751No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG83450.10628902412028478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT480150.069.508692
TGCTGGC175200.069.2370769
AGCTTAT348850.069.2145162
GTAGGTT423000.069.210168
AGTAGGT424000.069.19557
AGATGCT175000.069.176216
TGAGGTA599650.069.127311
CGGAGTC482800.069.1262914
TAGGTTG423300.069.119779
CTTCATT482650.069.082953
GGTAGTA587550.069.0660554
GGAGTCT482050.069.0549715
AGGTTGT442900.069.0479210
AAGATGC175350.068.997345
TAGTAGG424950.068.958456
CAAGATG175500.068.9583054
TTCATTC483200.068.9305654
GCTGGCA175800.068.88132510
TATAGTT387700.068.87128416
GTTGTAT324550.068.8638912
Produced by FastQC (version 0.11.5)