FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7896901
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG972141.2310398724765577No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC695720.881003826691002No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG423610.5364256181000623No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC333560.42239354399909534No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA312990.39634535117003494No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT298230.377654474837661No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG270290.34227350703776077No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA255700.3237979050262882No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC162630.20594154593048591No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA158760.20104088933114395No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC146150.18507259999840445No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA118420.14995755930079407No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA113940.14428444778527677No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT106850.1353062422841568No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT105790.13396394357736027No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA104540.13238104415896818No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC98050.12416263037867639No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG97200.12308625877416976No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG88550.11213259479889642No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA88470.11203128923611932No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA87130.110334421059603No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG84360.10682671594844612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT582950.069.616032
GTAGGTT599550.069.436798
AGTAGGT600900.069.414757
TAGGTTG599450.069.3899849
TGAGGTA838300.069.341051
AGCTTAT476250.069.331512
AAGATGC238550.069.29125
CGGAGTC586050.069.2690414
AGATGCT238500.069.263466
TAGTAGG601200.069.257866
TGCTGGC238600.069.24919
GGAGTCT584800.069.2401915
GGTAGTA825900.069.232654
AGGTTGT624700.069.22964510
CTTCATT585850.069.2159043
CCACCGG588000.069.1757910
TATAGTT539350.069.1700916
GTTGTAT455600.069.1611712
GTAGTAG820900.069.11295
AGGTAGT826100.069.104573
Produced by FastQC (version 0.11.5)