FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9482267
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1011491.0667174843315423No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG776480.8188759080502584No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG522560.5510918433324014No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA397020.41869734315644136No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC368140.3882404914352232No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG345040.36387922845876414No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC263600.27799259396513515No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA250590.26427224628878304No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT240280.2533993189603288No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA228570.24104995145148309No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG204630.21580282436678908No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT198900.2097599656284726No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA148680.15679794715757317No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC131930.1391333949993182No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA105450.11120758358734258No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG104470.11017407546106855No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT103860.10953076938246942No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA96590.10186382644572231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT599000.069.6246262
GTAGGTT740950.069.557088
AGTAGGT742950.069.487617
AGGTTGT767250.069.4489810
TAGGTTG742150.069.425769
GGTAGTA980400.069.4130254
TAGTAGG743800.069.389386
TGAGGTA995300.069.3834841
CTTCATT600950.069.3564763
TGCTGGC212000.069.303019
GTTGTAT534650.069.27630612
AGATGCT211650.069.235726
TATAGTT623000.069.2349216
AAGATGC211550.069.2173545
GGAGTCT601150.069.1947715
GTTGTGT242400.069.1733912
GGTTGTG242950.069.1320411
AGCTTAT466850.069.121442
GCTTATC466600.069.097013
AGGTAGT984000.069.072353
Produced by FastQC (version 0.11.5)