FastQCFastQC Report
Tue 28 May 2019
HGKN3BGXB_n01_HBH12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKN3BGXB_n01_HBH12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11232946
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1383821.2319297181701043No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC870480.7749347321708838No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA639750.5695300235574888No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG616880.5491702710936205No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA450910.40141740198875697No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA427500.38057692078284716No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT425370.37868071296701683No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC409190.3642766554739959No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG267830.23843255366846772No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC243350.21663951736258683No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC200160.1781901203833794No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC164180.14615934234883707No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT162920.14503764195074026No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA154180.1372569582369576No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT152840.13606403876596576No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA146930.130802729755845No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA146010.12998371041755208No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA134090.11937206855619177No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA130620.11628294126936958No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA121720.10835981940979687No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG121090.10779896921074845No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT119700.10656153781919721No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA115560.10287595079687911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT865850.069.594132
TGCTGGC314550.069.4954769
AGATGCT315300.069.463066
GTAGGTT767900.069.458378
AAGATGC315300.069.450425
AGTAGGT770450.069.418957
GGTAGTA1069400.069.316874
GCTGGCA315750.069.3089510
TAGGTTG769500.069.2957469
AGGTTGT802200.069.28503410
TGAGGTA1092600.069.2795261
CAAGATG315950.069.274314
CTTCATT869400.069.268473
GGAGTCT867200.069.263215
AGCTTAT626850.069.2166752
GTTGTAT591350.069.1912712
TAGTAGG772000.069.184376
TATAGTT707950.069.1081716
GGTTGTG221400.069.06351511
TTCATTC872050.069.019094
Produced by FastQC (version 0.11.5)